教大家如何做一篇meta-4

其明技术专家 meta分析, 博客, 基础科研 2017年5月3日

1.28W 0 1

NOS量表的制作

一、理论讲解

当我们提取完snp的原始文献的数据后，我们最好对原始文献进行质量评价（这是由病例-对照研究和cohort研究的设计特点决定的）。就是我们都来当判官，给文章打分和点赞，看他是否符合NOS，符合就点赞，给五星。

我们先看看例子：他汀类药物预防大肠癌，业内牛刊jco附件的质量评价表

Supplementary Table 3 Quality assessment of the included studies

Newcastle-Ottawa Scale for assessing the quality of studies in meta-analysis^

	Selection				Comparability	Outcome
Study	Represent	Selection of	Ascertain	Demonstration that	Comparability of	Assessme	Was follow-up		Qua
	ativeness	the non	ment of	outcome of interest	cohorts on the	nt of	long enough for	Adequacy	lity
	of the	exposed	exposure	was not present at	basis of the	outcome	outcomes to	of follow	scor
	exposed	cohort		start of study	design or		occur	up of	e
	cohort				analysis			cohorts
Krens et al,2014		★	★	★	★★	★	★	★	8
Ishikawa et al,2014		★	★	★	★★	★	★		7
Cardwell et al,2014		★	★	★	★	★	★		6
Zanders et al,2013		★	★	★	★	★	★	★	7
Mace et al,2013		★	★	★	★★	★	★		7
Ma et al,2013		★	★	★	★★	★	★	★	8
Nielsen et al,2012		★	★	★	★★	★	★	★	8
Lakha et al,2012		★	★	★	★★	★	★		7
Ng et al,2011		★	★	★	★★	★	★		7
Siddiqui et al,2009		★	★	★		★	★		5

Note: A study can be awarded a maximum of one star for each numbered item within the Selection and Outcome categories. A maximum of two stars can be given for Comparability.

猴哥自己文章的质量评价表

Table S1. Results of quality assessment using the Newcastle–Ottawa Scale for case-control studies

Study	Selection				Comparability	Exposure			Scores
	Adequate definition of cases	Representat- iveness of the cases	Selection of con- trols	Definition of con- trols	Control for important factor ^a	Ascertain- ment of exposure	Same method of ascertain- ment for cases and controls	Non- response rate
Kwang 2006	☆	☆	☆	☆	☆☆	☆	☆	-	8
Anvesha Srivastava 2008	☆	☆	☆	☆	☆☆	☆	☆	☆	9
Nopparat 2012	☆	☆	-	☆	☆☆	☆	☆	-	7
Li 2005	☆	☆	-	☆	☆	☆	☆	☆	7
Wang 2005	☆	☆	☆	☆	-	☆	☆	☆	7
Matsubayashi 2005	☆	☆	-	☆	☆	☆	☆	☆	7
Suzuk 2008	☆	☆	☆	☆	☆	☆	☆	☆	8
Yang 2009	☆	☆	☆	☆	-	☆	☆	☆	7
Ivan Nisevic 2008	☆	☆	☆	☆	☆☆	☆	☆	☆	9

^a A maximum of 2 stars can be allotted in this category, one for age, the other for other controlled factors.

二、原理

大家对照着表看看，好好琢磨琢磨，真正做meta的时候才能明白吧！！！这里列举猴哥收集的中文版的相关内容，睡觉的时候看看，是必备催眠佳品。

队列研究的NOS中文版

队列的选择：

（1）暴露队列的代表性：

很好的代表性 *
较好的代表性 *
代表性差，如选择志愿者、护士等
未描述队列的来源

（2）非暴露队列的选择：

与暴露队列来自同一人群，如同一社区 *
与暴露队列来自不同的人群
未描述来源

（3）暴露的确定：

严格确定的记录（如外科的记录） *
结构式问卷调查 *
自己的记录
未描述

（4）研究开始时没有研究对象已经发生研究的疾病：

是 *
否
可比性

暴露队列和非暴露队列的可比性（设计和分析阶段）

根据最重要的因素选择和分析对照 *
根据其他的重要因素（例如第二重要因素）选择和分析对照 *

（可以理解为是否对重要的混杂因素进行了校正）

结果
结果的测定方法：
独立的、盲法测定或评估 *
根据可靠地记录 *
自己的记录
未描述
对于所研究的疾病，随访时间是否足够长？
是的 *
否（时间太短，多数未发生所研究的疾病）
随访的完整性
随访完整，对所有的研究对象均随访到 *
随访率>80%（评价者自己可以确定一个合适的随访率），少数失访，失访小并对失访者进行了描述分析 *
随访率<80%，对失访者没有进行描述
未描述

NEWCASTLE - OTTAWA QUALITY ASSESSMENT SCALE FOR CASE CONTROL STUDIES

Note: A study can be awarded a maximum of one star for each numbered item within the Selection and Exposure categories. A maximum of two stars can be given for Comparability

Selection

1) Is the case definition adequate?

a) yes, with independent validation ¯
b) yes, eg record linkage or based on self reports
c) no description

2) Representativeness of the cases

a) consecutive or obviously representative series of cases ¯
b) potential for selection biases or not stated

3) Selection of Controls

a) community controls ¯
b) hospital controls
c) no description

4) Definition of Controls

a) no history of disease (endpoint) ¯
b) no description of source

Comparability

1) Comparability of cases and controls on the basis of the design or analysis

a) study controls for _______________ (Select the most important factor.) ¯
b) study controls for any additional factor ¯(This criteria could be modified to indicate specific control for a second important factor.)

Exposure

1) Ascertainment of exposure

a) secure record (eg surgical records) ¯
b) structured interview where blind to case/control status ¯
c) interview not blinded to case/control status
d) written self report or medical record only
e) no description

2) Same method of ascertainment for cases and controls

a) yes ¯
b) no

3) Non-Response rate

a) same rate for both groups ¯
b) non respondents described
c) rate different and no designation

三、实战分析

1.数据如下：具体指南如下：

下面几篇文章，我们的评分如下：

Study quality of included studies based on the Newcastle-Ottawa scale

3.1 Study quality of case-control studies

Author	Is the case definition adequate?	Representativeness of the Cases	Selection of Controls	Definition of Controls	Comparability of Cases and Controls on the Basis of the Design or Analysis	Ascertainment of exposure	Same method of ascertainment for cases and controls	Non-Response rate	Total scores
Elbendary MA 2009	★	★	★	★	☆☆	★	★	☆	6
Polsky JY 2005	★	★	☆	★	★★	★	★	☆	7
Zambon JP 2010	★	★	★	★	☆☆	★	★	☆	6
Zedan H 2010	★	★	☆	★	☆☆	★	★	☆	5

3.2 Study quality of cohort studies

Author	Representativeness of the exposed cohort	Selection of the non exposed cohort	Ascertainment of exposure	Demonstration that outcome of interest was not present at start of study	Comparability of cohorts on the basis of the design or analysis	Assessment of outcome	Was follow-up long enough for outcomes to occur	Adequacy of follow up of cohorts	Total scores
Bacon CG 2006	☆	★	★	★	★★	☆	★	★	8
Feldman HA 2000	★	★	★	★	★★	☆	☆	☆	6
Gades NM 2005	☆	★	★	★	★★	★	★	★	7
Shiri R 2005	★	★	★	★	★★	☆	☆	☆	6